Journal: Cells

Article Title: Participation of Extracellular Vesicles from Zika-Virus-Infected Mosquito Cells in the Modification of Naïve Cells’ Behavior by Mediating Cell-to-Cell Transmission of Viral Elements

doi: 10.3390/cells9010123

Figure Lengend Snippet: EVs from ZIKV-infected C6/36 modify towards a pro-coagulant, pro-inflammatory, and pro-adherent phenotype and favor permeability in naïve endothelial cells (ECs). ( A ) EC TF-1+ percentages (FACS) at different EVs stimuli conditions from three independent experiments. ( B ) EC Protease Activated Receptor+ (PAR-1) percentages at different EVs stimuli conditions from three independent experiments. ( C ) EC intercellular adhesion molecule-1+ (ICAM-1) percentages at different EVs stimuli conditions from three independent experiments. The TF, PAR-1, or ICAM-1 levels were compared (by an unpaired Student’s t-test) between all conditions’ values. ( D ) TNF-α mRNA expression (RT-PCR) in naïve ECs at different EVs stimuli conditions. The TNF-α genome conserved region (amplicon of 600 bp) was visualized on 2% ethidium bromide-stained 1.2% agarose gel. ( E ) Permeability percentages obtained by assessing the fluorescein isothiocyanate (FITC)-Dextran pass through the EC monolayers in the presence of different EV stimuli conditions. Three independent experiments were performed. For the 100% FITC-Dextran delivered control, a no-cell insert was used. The endothelial vascular permeability percentages were compared (by an unpaired Student’s t-test) between all conditions’ values. For all experiments, statistical significance was recognized as *, +, ¡, #, !,/, ~, or ° when p < 0.05, **, ++, ¡¡, ##, !!,//, ~~, or °° when p < 0.01, and ***, +++, ¡¡¡, ###, !!!,///, ~~~, or °°° when p < 0.0001.

Article Snippet: Likewise, the ECs were immunophenotyped with the mouse FITC-conjugated anti-human CD142 (TF) antibody (Catalog #13133-MM05-F, Sino Biological, USA), the mouse anti-Protease Activated Receptor (PAR-1) antibody (Catalog #sc-13503, Santa Cruz Biotechnology, USA), and the mouse FITC-conjugated anti-human CD54 (ICAM-1) antibody (Catalog # 35-0549-T025, Tonbo Biosciences, USA).

Techniques: Infection, Permeability, Expressing, Reverse Transcription Polymerase Chain Reaction, Amplification, Staining, Agarose Gel Electrophoresis, Control

Journal: Stem Cell Research & Therapy

Article Title: Human adipose tissue-derived stem cells cultured in xeno-free culture condition enhance c-MYC expression increasing proliferation but bypassing spontaneous cell transformation

doi: 10.1186/s13287-015-0030-4

Figure Lengend Snippet: Immunophenotypic analysis of human adipose tissue-derived stem cells. Flow cytometry analysis, where the dot plots represent the cell size and granularity of human adipose tissue-derived stem cells (hASCs) cultured in basal medium with (A) allogeneic human serum (aHS) and (B) fetal bovine serum (FBS). Expression of the selected mesenchymal stem cell, hematopoietic and human leukocyte antigens markers are depicted with representative histograms for (A) aHS cultures and (B) FBS cultures. The gray peak indicates the isotype-matched monoclonal antibody control. The black peak indicates positively stained cells. The cell populations expressed CD166, CD105, CD90, CD73, CD44, CD54 and HLA-ABC, and did not express CD45, CD34, CD19 and HLA-DR. (C-D) Bar graphs represent the quantitative analysis of the hASC expression pattern of markers in basal medium supplemented with (C) aHS and (D) FBS.

Article Snippet: The following antibodies were used: CD90, CD166, CD45, CD19, CD105-fluorescein isothiocyanate (FITC), CD44 (HCAM), CD73-phycoerythrin (all from BD Biosciences, USA), CD54-FITC (ICAM; Caltag Medsystems, UK), CD34 (Santa Cruz Biotechnology, USA), HLA-ABC-FITC and HLA-DR-FITC (Abcam, USA).

Techniques: Derivative Assay, Flow Cytometry, Cell Culture, Expressing, Staining